Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples
Identifieur interne : 000786 ( Main/Exploration ); précédent : 000785; suivant : 000787Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples
Auteurs : Eric A. E. Garber [États-Unis] ; Joseph TholeSource :
- Toxins [ 2072-6651 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Gliadin, Ricin.
- analysis : Bread, Carbonated Beverages, Food Contamination, Fruit and Vegetable Juices.
- chemistry : Milk.
- methods : Enzyme-Linked Immunosorbent Assay.
- Animals, Citrus sinensis, Hot Temperature, Microwaves, Toxins, Biological.
Abstract
The utility of microwave irradiation to accelerate the onset of equilibrium and improve ELISA performance was examined using ELISAs for the detection of the plant toxin ricin and gliadin. The ricin ELISA normally requires several one hour incubations at 37 °C, a total assay time of approximately five hours, and employs a complex buffer containing PBS, Tween-20®, and non-fat milk. Different energy levels and pulse designs were compared to the use of abbreviated incubation times at 37 °C for the detection of ricin in food. The use of microwave irradiation had no significant advantage over the application of heat using an oven incubator and performed worse with some foods. In contrast, a gliadin ELISA that relied on 30 min incubation steps at room temperature and a salt-based buffer performed better upon irradiation but also displayed improvement upon incubating the microtiter plate at 37 °C. Whether microwave irradiation was advantageous compared to incubation in an oven was inconclusive. However, by abbreviating the incubation time of the ricin ELISA, it was possible to cut the assay time to less than 2 hours and still display LOD values < 10 ppb and recoveries of 78%–98%.
Url:
DOI: 10.3390/toxins7062135
PubMed: 26110503
PubMed Central: 4488694
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples </title><author><name sortKey="Garber, Eric A E" sort="Garber, Eric A E" uniqKey="Garber E" first="Eric A. E." last="Garber">Eric A. E. Garber</name><affiliation wicri:level="1"><nlm:aff id="af1-toxins-07-02135">Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740</wicri:regionArea><wicri:noRegion>MD 20740</wicri:noRegion></affiliation></author><author><name sortKey="Thole, Joseph" sort="Thole, Joseph" uniqKey="Thole J" first="Joseph" last="Thole">Joseph Thole</name><affiliation><nlm:aff id="af2-toxins-07-02135">Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742, USA; E-Mail:<email>jthole@terpmail.umd.edu</email></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26110503</idno><idno type="pmc">4488694</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488694</idno><idno type="RBID">PMC:4488694</idno><idno type="doi">10.3390/toxins7062135</idno><date when="2015">2015</date><idno type="wicri:Area/Pmc/Corpus">000238</idno><idno type="wicri:Area/Pmc/Curation">000237</idno><idno type="wicri:Area/Pmc/Checkpoint">000604</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">000166</idno><idno type="wicri:Area/PubMed/Curation">000166</idno><idno type="wicri:Area/PubMed/Checkpoint">000166</idno><idno type="wicri:Area/Ncbi/Merge">001B91</idno><idno type="wicri:Area/Ncbi/Curation">001B91</idno><idno type="wicri:Area/Ncbi/Checkpoint">001B91</idno><idno type="wicri:Area/Main/Merge">000786</idno><idno type="wicri:Area/Main/Curation">000786</idno><idno type="wicri:Area/Main/Exploration">000786</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Application of Microwave Irradiation and Heat to Improve Gliadin Detection and Ricin ELISA Throughput with Food Samples </title><author><name sortKey="Garber, Eric A E" sort="Garber, Eric A E" uniqKey="Garber E" first="Eric A. E." last="Garber">Eric A. E. Garber</name><affiliation wicri:level="1"><nlm:aff id="af1-toxins-07-02135">Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD 20740</wicri:regionArea><wicri:noRegion>MD 20740</wicri:noRegion></affiliation></author><author><name sortKey="Thole, Joseph" sort="Thole, Joseph" uniqKey="Thole J" first="Joseph" last="Thole">Joseph Thole</name><affiliation><nlm:aff id="af2-toxins-07-02135">Joint Institute for Food Safety and Applied Nutrition (JIFSAN), University of Maryland, College Park, MD 20742, USA; E-Mail:<email>jthole@terpmail.umd.edu</email></nlm:aff></affiliation></author></analytic><series><title level="j">Toxins</title><idno type="eISSN">2072-6651</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bread (analysis)</term><term>Carbonated Beverages (analysis)</term><term>Citrus sinensis</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Food Contamination (analysis)</term><term>Fruit and Vegetable Juices (analysis)</term><term>Gliadin (analysis)</term><term>Hot Temperature</term><term>Microwaves</term><term>Milk (chemistry)</term><term>Ricin (analysis)</term><term>Toxins, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Gliadin</term><term>Ricin</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Bread</term><term>Carbonated Beverages</term><term>Food Contamination</term><term>Fruit and Vegetable Juices</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Milk</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Citrus sinensis</term><term>Hot Temperature</term><term>Microwaves</term><term>Toxins, Biological</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The utility of microwave irradiation to accelerate the onset of equilibrium and improve ELISA performance was examined using ELISAs for the detection of the plant toxin ricin and gliadin. The ricin ELISA normally requires several one hour incubations at 37 °C, a total assay time of approximately five hours, and employs a complex buffer containing PBS, Tween-20<sup>®</sup>, and non-fat milk. Different energy levels and pulse designs were compared to the use of abbreviated incubation times at 37 °C for the detection of ricin in food. The use of microwave irradiation had no significant advantage over the application of heat using an oven incubator and performed worse with some foods. In contrast, a gliadin ELISA that relied on 30 min incubation steps at room temperature and a salt-based buffer performed better upon irradiation but also displayed improvement upon incubating the microtiter plate at 37 °C. Whether microwave irradiation was advantageous compared to incubation in an oven was inconclusive. However, by abbreviating the incubation time of the ricin ELISA, it was possible to cut the assay time to less than 2 hours and still display LOD values < 10 ppb and recoveries of 78%–98%.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="He, J" uniqKey="He J">J. He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Dorp, R" uniqKey="Van Dorp R">R. Van Dorp</name></author><author><name sortKey="Daha, M R" uniqKey="Daha M">M.R. Daha</name></author><author><name sortKey="Muizert, Y" uniqKey="Muizert Y">Y. Muizert</name></author><author><name sortKey="Marani, E" uniqKey="Marani E">E. Marani</name></author><author><name sortKey="Boon, M E" uniqKey="Boon M">M.E. Boon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gu, Q" uniqKey="Gu Q">Q. Gu</name></author><author><name sortKey="Zhang, H" uniqKey="Zhang H">H. 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Sharma</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Thole, Joseph" sort="Thole, Joseph" uniqKey="Thole J" first="Joseph" last="Thole">Joseph Thole</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Garber, Eric A E" sort="Garber, Eric A E" uniqKey="Garber E" first="Eric A. E." last="Garber">Eric A. E. Garber</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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